› 7b7h

X-ray diffraction
1.8Å resolution

The glucuronoyl esterase OtCE15A R268A variant from Opitutus terrae in complex with, and covalently linked to, D-glucuronate

Released:
DOI: 10.2210/pdb7b7h/pdb
PDB entry 7b7h coloured by chain, front view.
PDB entry 7b7h coloured by chain, side view.
PDB entry 7b7h coloured by chain, top view.
Source organism: Opitutus terrae PB90-1
Primary publication:
Mechanism and biomass association of glucuronoyl esterase: an α/β hydrolase with potential in biomass conversion.
Zong Z, Mazurkewich S, Pereira CS, Fu H, Cai W, Shao X, Skaf MS, Larsbrink J, Lo Leggio L
Nat Commun 13 1449 (2022)
PMID: 35304453

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative acetyl xylan esterase Chain: A
Molecule details ›
Chain: A
Length: 421 amino acids
Theoretical weight: 46.06 KDa
Source organism: Opitutus terrae PB90-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1ZMF4 (Residues: 33-432; Coverage: 96%)
Gene name: Oter_0116

Ligands and Environments

5 bound ligands:
Ligand GCU 1 x GCU
Ligand DMS 11 x DMS
Ligand NA 2 x NA
Ligand EDO 12 x EDO
Ligand GLY 4 x GLY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P1
Unit cell:
a: 47.333Å b: 47.298Å c: 51.112Å
α: 61.947° β: 67.92° γ: 88.272°
R-values:
R R work R free
0.185 0.183 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Glucuronoyl esterases - enzymes to decouple lignin and carbohydrates and enable better utilization of renewable plant biomass.
Larsbrink et al. (2023)
1 more

1 mention without citation

Mechanism and biomass association of glucuronoyl esterase: an α/β hydrolase with potential in biomass conversion.
Zong et al. (2022)