› 7b7q

X-ray diffraction
1.35Å resolution

CooS-V with oxidized hybrid cluster

Released:
DOI: 10.2210/pdb7b7q/pdb
PDB entry 7b7q coloured by chain, front view.
PDB entry 7b7q coloured by chain, side view.
PDB entry 7b7q coloured by chain, top view.
Source organism: Carboxydothermus hydrogenoformans Z-2901
Primary publication:
A Morphing [4Fe-3S-nO]-Cluster within a Carbon Monoxide Dehydrogenase Scaffold.
Jeoung JH, Fesseler J, Domnik L, Klemke F, Sinnreich M, Teutloff C, Dobbek H
Angew Chem Int Ed Engl 61 e202117000 (2022)
PMID: 35133707

Function and Biology Details

Reaction catalysed: 
CO + H(2)O + 2 oxidized ferredoxin = CO(2) + 2 reduced ferredoxin + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbon monoxide dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 629 amino acids
Theoretical weight: 68.32 KDa
Source organism: Carboxydothermus hydrogenoformans Z-2901
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3AG28 (Residues: 1-629; Coverage: 100%)
Gene names: CHY_0034, cooSV
Sequence domains: Prismane/CO dehydrogenase family

Ligands and Environments

5 bound ligands:
Ligand FES 1 x FES
Ligand SF4 2 x SF4
Ligand T2N 2 x T2N
Ligand TAM 1 x TAM
Ligand PEG 2 x PEG
1 modified residue:
Ligand CSS 2 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 66.327Å b: 108.982Å c: 82.843Å
α: 90° β: 98.78° γ: 90°
R-values:
R R work R free
0.133 0.133 0.158
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

A Morphing [4Fe-3S-nO]-Cluster within a Carbon Monoxide Dehydrogenase Scaffold.
Jeoung et al. (2022)
2 more