› 7b9a

X-ray diffraction
2.5Å resolution

CooS-V with Xe-soaked

Released:
DOI: 10.2210/pdb7b9a/pdb
PDB entry 7b9a coloured by chain, front view.
PDB entry 7b9a coloured by chain, side view.
PDB entry 7b9a coloured by chain, top view.
Source organism: Carboxydothermus hydrogenoformans Z-2901
Primary publication:
A Morphing [4Fe-3S-nO]-Cluster within a Carbon Monoxide Dehydrogenase Scaffold.
Jeoung JH, Fesseler J, Domnik L, Klemke F, Sinnreich M, Teutloff C, Dobbek H
Angew Chem Int Ed Engl 61 e202117000 (2022)
PMID: 35133707

Function and Biology Details

Reaction catalysed: 
CO + H(2)O + 2 oxidized ferredoxin = CO(2) + 2 reduced ferredoxin + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbon monoxide dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 629 amino acids
Theoretical weight: 68.29 KDa
Source organism: Carboxydothermus hydrogenoformans Z-2901
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3AG28 (Residues: 1-629; Coverage: 100%)
Gene names: CHY_0034, cooSV
Sequence domains: Prismane/CO dehydrogenase family

Ligands and Environments

6 bound ligands:
Ligand SF4 4 x SF4
Ligand XE 61 x XE
Ligand BR 23 x BR
Ligand T2N 4 x T2N
Ligand H2S 3 x H2S
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 69.41Å b: 230.05Å c: 82.74Å
α: 90° β: 101.52° γ: 90°
R-values:
R R work R free
0.238 0.236 0.276
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

A Morphing [4Fe-3S-nO]-Cluster within a Carbon Monoxide Dehydrogenase Scaffold.
Jeoung et al. (2022)
2 more