› 7b9p

X-ray diffraction
2.65Å resolution

Structure of Ribonucleotide reductase from Rhodobacter sphaeroides

Released:
DOI: 10.2210/pdb7b9p/pdb
PDB entry 7b9p coloured by chain, front view.
PDB entry 7b9p coloured by chain, side view.
PDB entry 7b9p coloured by chain, top view.
Source organism: Cereibacter sphaeroides 2.4.1
Primary publication:
HUG Domain Is Responsible for Active Dimer Stabilization in an NrdJd Ribonucleotide Reductase.
Fietze T, Wilk P, Kabinger F, Anoosheh S, Hofer A, Lundin D, Feiler CG, Weiss MS, Loderer C
Biochemistry (2022)
PMID: 35856337

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vitamin B12-dependent ribonucleotide reductase Chain: A
Molecule details ›
Chain: A
Length: 925 amino acids
Theoretical weight: 100.52 KDa
Source organism: Cereibacter sphaeroides 2.4.1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3J3H6 (Residues: 1-925; Coverage: 76%)
Gene names: RSP_2495, nrd
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand DTP 1 x DTP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6522
Unit cell:
a: 140.748Å b: 140.748Å c: 364.277Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.23 0.272
Expression system: Escherichia coli BL21(DE3)

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