› 7bg9

Electron Microscopy
3.8Å resolution

The catalytic core lobe of human telomerase in complex with a telomeric DNA substrate

Released:
DOI: 10.2210/pdb7bg9/pdb
PDB entry 7bg9 coloured by chain, front view.
PDB entry 7bg9 coloured by chain, side view.
PDB entry 7bg9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human telomerase holoenzyme with bound telomeric DNA.
Ghanim GE, Fountain AJ, van Roon AM, Rangan R, Das R, Collins K, Nguyen THD
Nature 593 449-453 (2021)
PMID: 33883742
Related structures: EMD-12174

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-109984 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (5 distinct):
Telomerase reverse transcriptase Chain: A
Molecule details ›
Chain: A
Length: 1332 amino acids
Theoretical weight: 149.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O14746 (Residues: 1-1132; Coverage: 100%)
Gene names: EST2, TCS1, TERT, TRT
Sequence domains:
Histone H2B Chain: M
Molecule details ›
Chain: M
Length: 166 amino acids
Theoretical weight: 18.07 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: B4DR52 (Residues: 1-166; Coverage: 100%)
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H2A type 1 Chain: L
Molecule details ›
Chain: L
Length: 130 amino acids
Theoretical weight: 14.14 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0C0S8 (Residues: 1-130; Coverage: 100%)
Gene names: H2AC11, H2AC13, H2AC15, H2AC16, H2AC17, H2AFC, H2AFD, H2AFI, H2AFN, H2AFP, HIST1H2AG, HIST1H2AI, HIST1H2AK, HIST1H2AL, HIST1H2AM
Sequence domains:
DNA (5'-D(P*TP*TP*AP*GP*GP*G)-3') Chain: N
Molecule details ›
Chain: N
Length: 18 nucleotides
Theoretical weight: 5.51 KDa
RNA (256-MER) Chain: B
Molecule details ›
Chain: B
Length: 451 nucleotides
Theoretical weight: 145.48 KDa
Sequence domains: Vertebrate telomerase RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-12174
Expression system: Homo sapiens

Quick links

Citations

17 review citations

Targeting telomeres: advances in telomere maintenance mechanism-specific cancer therapies.
Gao et al. (2022)
16 more

7 mentions without citation

Advances in understanding telomerase assembly.
Klump et al. (2023)
6 more