› 7bhy

X-ray diffraction
2.3Å resolution

DNA-binding domain of DeoR in complex with the DNA operator

Released:
DOI: 10.2210/pdb7bhy/pdb
PDB entry 7bhy coloured by chain, front view.
PDB entry 7bhy coloured by chain, side view.
PDB entry 7bhy coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural insight into DNA recognition by bacterial transcriptional regulators of the SorC/DeoR family.
Šoltysová M, Sieglová I, Fábry M, Brynda J, Škerlová J, Řezáčová P
Acta Crystallogr D Struct Biol 77 1411-1424 (2021)
PMID: 34726169

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-121115 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Deoxyribonucleoside regulator Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 57 amino acids
Theoretical weight: 6.6 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39140 (Residues: 4-55; Coverage: 17%)
Gene names: BSU39430, deoR, yxxC
Sequence domains: Homeodomain-like domain
DNA operator - strand 1 Chain: E
Molecule details ›
Chain: E
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Not provided
DNA operator - strand 2 Chain: G
Molecule details ›
Chain: G
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P42212
Unit cell:
a: 96.836Å b: 96.836Å c: 81.966Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 citation in other articles

Structural analysis of Red1 as a conserved scaffold of the RNA-targeting MTREC/PAXT complex.
Foucher et al. (2022)
1 more