› 7bil

X-ray diffraction
2.21Å resolution

Crystal structure of helicase Pif1 from Thermus oshimai in complex with oligo GGTTTGGTTTGGTT

Released:
DOI: 10.2210/pdb7bil/pdb
PDB entry 7bil coloured by chain, front view.
PDB entry 7bil coloured by chain, side view.
PDB entry 7bil coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional studies of SF1B Pif1 from Thermus oshimai reveal dimerization-induced helicase inhibition.
Dai YX, Chen WF, Liu NN, Teng FY, Guo HL, Hou XM, Dou SX, Rety S, Xi XG
Nucleic Acids Res 49 4129-4143 (2021)
PMID: 33784404

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121117 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
PIF1 helicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 507 amino acids
Theoretical weight: 57.05 KDa
Source organism: Thermus oshimai JL-2
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: K7RJ88 (Residues: 1-507; Coverage: 100%)
Gene name: Theos_1468
Sequence domains: PIF1-like helicase
DNA (5'-D(*GP*GP*TP*TP*TP*GP*GP*TP*TP*TP*GP*GP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.36 KDa
Source organism: Thermus oshimai
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand ALF 2 x ALF
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2221
Unit cell:
a: 88.052Å b: 103.438Å c: 251.566Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.22
Expression systems:
  • Escherichia coli K-12
  • Not provided

Quick links

Citations

4 review citations

G-quadruplex DNA: a novel target for drug design.
Teng et al. (2021)
3 more

2 mentions without citation

Structural and functional studies of SF1B Pif1 from Thermus oshimai reveal dimerization-induced helicase inhibition.
Dai et al. (2021)
1 more