› 7biv

X-ray diffraction
1.64Å resolution

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Mdm2 Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 11.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00987 (Residues: 17-109; Coverage: 19%)
Gene name: MDM2
Sequence domains: SWIB/MDM2 domain

Ligands and Environments

4 bound ligands:
Ligand DMS 2 x DMS
Ligand EDO 1 x EDO
Ligand SO4 2 x SO4
Ligand TUW 1 x TUW
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6522
Unit cell:
a: 71.646Å b: 71.646Å c: 100.483Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.227 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Abrogating the Interaction Between p53 and Mortalin (Grp75/HSPA9/mtHsp70) for Cancer Therapy: The Story so far.
Elwakeel A. (2022)
2 more

2 mentions without citation

Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak.
Ivanovitch et al. (2021)
1 more