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› 7bjk

X-ray diffraction
2.25Å resolution

Crystal structure of the chloroplastic Fe superoxide dismutase PAP9 from Arabidopsis thaliana.

Released:
DOI: 10.2210/pdb7bjk/pdb
Model geometry
Fit model/data
PDB entry 7bjk coloured by chain, front view.
PDB entry 7bjk coloured by chain, side view.
PDB entry 7bjk coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The Plastid-Encoded RNA Polymerase-Associated Protein PAP9 Is a Superoxide Dismutase With Unusual Structural Features.
Favier A, Gans P, Boeri Erba E, Signor L, Muthukumar SS, Pfannschmidt T, Blanvillain R, Cobessi D
OpenAccess logo Front Plant Sci 12 668897 (2021)
PMID: 34276730

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = H2O2 + O2.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Fe] 2, chloroplastic Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 270 amino acids
Theoretical weight: 30.88 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LU64 (Residues: 47-305; Coverage: 85%)
Gene names: APG8, At5g51100, FSD2, MWD22.4
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 214.09Å b: 83.01Å c: 118.24Å
α: 90° β: 115.759° γ: 90°
R-values:
R R work R free
0.181 0.179 0.221
Expression system: Escherichia coli

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Citations

1 review citation

Chloroplast gene expression: Recent advances and perspectives.
Zhang et al. (2023)
3 other articles

2 mentions without citation

The Plastid-Encoded RNA Polymerase-Associated Protein PAP9 Is a Superoxide Dismutase With Unusual Structural Features.
Favier et al. (2021)
1 more