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› 7bkx

X-ray diffraction
2.35Å resolution

Diploptera punctata inspired lipocalin-like Milk protein expressed in Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb7bkx/pdb
Model geometry
Fit model/data
PDB entry 7bkx coloured by chain, front view.
PDB entry 7bkx coloured by chain, side view.
PDB entry 7bkx coloured by chain, top view.
Source organism: Diploptera punctata
Primary publication:
Structure of recombinantly expressed cockroach Lili-Mip protein in glycosylated and deglycosylated forms.
KanagaVijayan D, Subramanian R, Santhakumari PR, Chavas LMG, Subramanian R, Banerjee S
Biochim Biophys Acta Gen Subj 1866 130064 (2022)
PMID: 34958847

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Milk protein Chain: AAA
Molecule details ›
Chain: AAA
Length: 177 amino acids
Theoretical weight: 20.64 KDa
Source organism: Diploptera punctata
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q6SVB5 (Residues: 10-164; Coverage: 95%)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 3 x NAG
Ligand PAM 1 x PAM
Ligand GOL 1 x GOL
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 51.61Å b: 135.662Å c: 39.654Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.278
Expression system: Saccharomyces cerevisiae

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Citations

1 mention without citation

Variability in phenylalanine side chain conformations facilitates broad substrate tolerance of fatty acid binding in cockroach milk proteins.
Santhakumari et al. (2023)