› 7bnt

X-ray diffraction
1.32Å resolution

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikD with a predicted ancestral HMA domain of Pik-1 from Oryza spp.

Released:
DOI: 10.2210/pdb7bnt/pdb
PDB entry 7bnt coloured by chain, front view.
PDB entry 7bnt coloured by chain, side view.
PDB entry 7bnt coloured by chain, top view.
Source organisms:
Primary publication:
Two NLR immune receptors acquired high-affinity binding to a fungal effector through convergent evolution of their integrated domain.
Białas A, Langner T, Harant A, Contreras MP, Stevenson CE, Lawson DM, Sklenar J, Kellner R, Moscou MJ, Terauchi R, Banfield MJ, Kamoun S
Elife 10 (2021)
PMID: 34288868

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111283 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Predicted ancestral HMA domain of Pik-1 from Oryza spp. Chains: A, B
Molecule details ›
Chains: A, B
Length: 75 amino acids
Theoretical weight: 7.8 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
AVR-Pik protein Chain: C
Molecule details ›
Chain: C
Length: 94 amino acids
Theoretical weight: 10.85 KDa
Source organism: Pyricularia oryzae
Expression system: Escherichia coli
UniProt:
  • Canonical: C4B8B8 (Residues: 22-113; Coverage: 100%)
Gene names: AVR-Pik, AvrPik, Pikm, Pikp

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P41212
Unit cell:
a: 119.486Å b: 119.486Å c: 35.974Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.145 0.184
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Molecular insights into the biochemical functions and signalling mechanisms of plant NLRs.
Liu et al. (2022)
3 more

1 mention without citation

Exploiting Structural Modelling Tools to Explore Host-Translocated Effector Proteins.
Amoozadeh et al. (2021)