› 7bru

X-ray diffraction
2.15Å resolution

Crystal structure of human RTN3 LIR fused to human GABARAP

Released:
DOI: 10.2210/pdb7bru/pdb
PDB entry 7bru coloured by chain, front view.
PDB entry 7bru coloured by chain, side view.
PDB entry 7bru coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Super-assembly of ER-phagy receptor Atg40 induces local ER remodeling at contacts with forming autophagosomal membranes.
Mochida K, Yamasaki A, Matoba K, Kirisako H, Noda NN, Nakatogawa H
Nat Commun 11 3306 (2020)
PMID: 32620754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-aminobutyric acid receptor-associated protein; Reticulon-3 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 138 amino acids
Theoretical weight: 16.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95197 (Residues: 244-264; Coverage: 2%)
  • Canonical: O95166 (Residues: 1-116; Coverage: 99%)
Gene names: ASYIP, FLC3B, GABARAP, HT004, NSPL2, RTN3
Sequence domains: Autophagy protein Atg8 ubiquitin like

Ligands and Environments

1 bound ligand:
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P41212
Unit cell:
a: 137.418Å b: 137.418Å c: 67.444Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

ER-Phagy, ER Homeostasis, and ER Quality Control: Implications for Disease.
Ferro-Novick et al. (2021)
9 more