› 7bu6

X-ray diffraction
2.7Å resolution

Structure of human beta1 adrenergic receptor bound to norepinephrine and nanobody 6B9

Released:
DOI: 10.2210/pdb7bu6/pdb
Model geometry
Fit model/data
PDB entry 7bu6 coloured by chain, front view.
PDB entry 7bu6 coloured by chain, side view.
PDB entry 7bu6 coloured by chain, top view.
Source organisms:
Primary publication:
Binding pathway determines norepinephrine selectivity for the human β1AR over β2AR.
Xu X, Kaindl J, Clark MJ, Hübner H, Hirata K, Sunahara RK, Gmeiner P, Kobilka BK, Liu X
Cell Res 31 569-579 (2021)
PMID: 33093660

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207764 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-1 adrenergic receptor; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 516 amino acids
Theoretical weight: 57.63 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: D9IEF7 (Residues: 2-161; Coverage: 98%)
  • Canonical: P08588 (Residues: 54-399; Coverage: 73%)
Gene names: ADRB1, ADRB1R, B1AR, T4Tp126, e
Sequence domains:
camelid antibody fragment Chain: B
Molecule details ›
Chain: B
Length: 128 amino acids
Theoretical weight: 14.02 KDa
Source organism: Camelidae mixed library
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
7 bound ligands:
Ligand E5E 1 x E5E
Ligand SO4 7 x SO4
Ligand NA 1 x NA
Ligand EPE 1 x EPE
Ligand 480 1 x 480
Ligand CLR 1 x CLR
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2
Unit cell:
a: 376.779Å b: 66.009Å c: 47.841Å
α: 90° β: 93.458° γ: 90°
R-values:
R R work R free
0.233 0.232 0.258
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

16 review citations

Short-Chain Fatty Acid Receptors and Cardiovascular Function.
Lymperopoulos et al. (2022)
15 more

5 mentions without citation

Tilianin: A Potential Natural Lead Molecule for New Drug Design and Development for the Treatment of Cardiovascular Disorders.
Khattulanuar et al. (2022)
4 more