› 7bui

X-ray diffraction
2.15Å resolution

Complex of reduced oxygenase and oxidized ferredoxin in carbazole 1,9a- dioxygenase

Released:
DOI: 10.2210/pdb7bui/pdb
Model geometry
Fit model/data
PDB entry 7bui coloured by chain, front view.
PDB entry 7bui coloured by chain, side view.
PDB entry 7bui coloured by chain, top view.
Source organisms:
Entry authors: Matsuzawa J, Wang YX, Suzuki-Minakuchi C, Nojiri H

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183052 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rieske domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 392 amino acids
Theoretical weight: 44.91 KDa
Source organism: Janthinobacterium sp. J3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q84II6 (Residues: 1-384; Coverage: 100%)
Gene name: carAa
Sequence domains:
Ferredoxin CarAc Chains: D, E
Molecule details ›
Chains: D, E
Length: 115 amino acids
Theoretical weight: 12.45 KDa
Source organism: Metapseudomonas resinovorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GI16 (Residues: 1-107; Coverage: 100%)
Gene name: carAc
Sequence domains: Rieske [2Fe-2S] domain

Ligands and Environments

4 bound ligands:
Ligand FES 5 x FES
Ligand FE2 3 x FE2
Ligand EDO 10 x EDO
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 97.29Å b: 81.56Å c: 116.13Å
α: 90° β: 100.12° γ: 90°
R-values:
R R work R free
0.19 0.187 0.238
Expression system: Escherichia coli BL21(DE3)

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