› 7bv6

X-ray diffraction
3.05Å resolution

Crystal structure of the autophagic STX17/SNAP29/VAMP8 SNARE complex

Released:
DOI: 10.2210/pdb7bv6/pdb
PDB entry 7bv6 coloured by chain, front view.
PDB entry 7bv6 coloured by chain, side view.
PDB entry 7bv6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Decoding three distinct states of the Syntaxin17 SNARE motif in mediating autophagosome-lysosome fusion.
Li Y, Cheng X, Li M, Wang Y, Fu T, Zhou Z, Wang Y, Gong X, Xu X, Liu J, Pan L
Proc Natl Acad Sci U S A 117 21391-21402 (2020)
PMID: 32817423

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-132004 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Vesicle-associated membrane protein 8 Chains: A, E, I, M, Q, U
Molecule details ›
Chains: A, E, I, M, Q, U
Length: 68 amino acids
Theoretical weight: 7.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BV40 (Residues: 8-75; Coverage: 68%)
Gene name: VAMP8
Sequence domains: Synaptobrevin
Syntaxin-17 Chains: B, F, J, N, R, V
Molecule details ›
Chains: B, F, J, N, R, V
Length: 87 amino acids
Theoretical weight: 9.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P56962 (Residues: 142-228; Coverage: 29%)
Gene name: STX17
Synaptosomal-associated protein 29 Chains: C, G, K, O, S, W
Molecule details ›
Chains: C, G, K, O, S, W
Length: 91 amino acids
Theoretical weight: 10.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95721 (Residues: 40-130; Coverage: 35%)
Gene name: SNAP29
Synaptosomal-associated protein 29 Chains: D, H, L, P, T, X
Molecule details ›
Chains: D, H, L, P, T, X
Length: 68 amino acids
Theoretical weight: 7.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95721 (Residues: 191-258; Coverage: 26%)
Gene name: SNAP29

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21212
Unit cell:
a: 143.981Å b: 196Å c: 76.142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.175 0.255
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Mitochondrial Fission Protein 1: Emerging Roles in Organellar Form and Function in Health and Disease.
Ihenacho et al. (2021)
2 more