› 7bvb

X-ray diffraction
3.19Å resolution

Crystal structure of UDP-N-acetylmuramic Acid L-alanine ligase (MurC) from Mycobacterium bovis in complex with UDP-N-acetylglucosamine

Released:
DOI: 10.2210/pdb7bvb/pdb
Model geometry
Fit model/data
PDB entry 7bvb coloured by chain, front view.
PDB entry 7bvb coloured by chain, side view.
PDB entry 7bvb coloured by chain, top view.
Source organism: Mycobacterium tuberculosis variant bovis AF2122/97
Primary publication:
Crystal structures of UDP-N-acetylmuramic acid L-alanine ligase (MurC) from Mycobacterium bovis with and without UDP-N-acetylglucosamine.
Seo PW, Park SY, Hofmann A, Kim JS
Acta Crystallogr D Struct Biol 77 618-627 (2021)
PMID: 33950018

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramate--L-alanine ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 54.4 KDa
Source organism: Mycobacterium tuberculosis variant bovis AF2122/97
Expression system: Escherichia coli
UniProt:
  • Canonical: P65473 (Residues: 1-494; Coverage: 100%)
Gene names: BQ2027_MB2176C, murC
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand UD1 2 x UD1
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 11C
Spacegroup: P21
Unit cell:
a: 65.285Å b: 75.762Å c: 104.679Å
α: 90° β: 105.92° γ: 90°
R-values:
R R work R free
0.198 0.195 0.247
Expression system: Escherichia coli

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Citations

1 mention without citation

Morphology of Lymphoid Tissue in the Lungs of Guinea Pigs Infected with Mycobacterium bovis against the Background of Vaccine Immunity and the Action of Betulin and Its Derivatives.
Koshkin et al. (2022)