› 7bw0

Electron Microscopy
3.9Å resolution

Active human TGR5 complex with a synthetic agonist 23H

Released:
DOI: 10.2210/pdb7bw0/pdb
PDB entry 7bw0 coloured by chain, front view.
PDB entry 7bw0 coloured by chain, side view.
PDB entry 7bw0 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of activated bile acids receptor TGR5 in complex with stimulatory G protein.
Chen G, Wang X, Ge Y, Ma L, Chen Q, Liu H, Du Y, Ye RD, Hu H, Ren R
Signal Transduct Target Ther 5 142 (2020)
PMID: 32747649
Related structures: EMD-30221

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209561 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
G-protein coupled bile acid receptor 1; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 427 amino acids
Theoretical weight: 46.18 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
  • Canonical: Q8TDU6 (Residues: 6-307; Coverage: 92%)
Gene names: GPBAR1, TGR5, cybC
Sequence domains:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 43.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 26-394; Coverage: 90%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 357 amino acids
Theoretical weight: 39.29 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody Nb35 Chain: N
Molecule details ›
Chain: N
Length: 157 amino acids
Theoretical weight: 17.06 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-30221
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

2 review citations

GPCR-mediated effects of fatty acids and bile acids on glucose homeostasis.
Oteng et al. (2023)
1 more

3 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
2 more