› 7bzd

X-ray diffraction
2.61Å resolution

Structure of Bacillus subtilis HxlR, wild type

Released:
DOI: 10.2210/pdb7bzd/pdb
PDB entry 7bzd coloured by chain, front view.
PDB entry 7bzd coloured by chain, side view.
PDB entry 7bzd coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Genetically encoded formaldehyde sensors inspired by a protein intra-helical crosslinking reaction.
Zhu R, Zhang G, Jing M, Han Y, Li J, Zhao J, Li Y, Chen PR
Nat Commun 12 581 (2021)
PMID: 33495458

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154644 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional activator HxlR Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 14.41 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42406 (Residues: 1-120; Coverage: 100%)
Gene names: BSU03470, hxlR
Sequence domains: HxlR-like helix-turn-helix

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: I222
Unit cell:
a: 53.161Å b: 60.772Å c: 70.681Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.241 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The cellular function and molecular mechanism of formaldehyde in cardiovascular disease and heart development.
Zhang et al. (2021)
3 more