› 7c1a

X-ray diffraction
2.14Å resolution

Crystal structure of FumaraseC (S319C) from Mannheimia succiniciproducens in complex with malate

Released:
DOI: 10.2210/pdb7c1a/pdb
PDB entry 7c1a coloured by chain, front view.
PDB entry 7c1a coloured by chain, side view.
PDB entry 7c1a coloured by chain, top view.
Source organism: [Mannheimia] succiniciproducens MBEL55E
Entry authors: Kim B, Lee SH, Kim K-J

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179229 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase class II Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 467 amino acids
Theoretical weight: 50.29 KDa
Source organism: [Mannheimia] succiniciproducens MBEL55E
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q65UJ3 (Residues: 1-465; Coverage: 100%)
Gene names: MS0760, fumC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand LMR 4 x LMR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 62.592Å b: 106.238Å c: 125.317Å
α: 90° β: 94.98° γ: 90°
R-values:
R R work R free
0.185 0.182 0.232
Expression system: Escherichia coli K-12

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