› 7c2b

X-ray diffraction
1.79Å resolution

Crystal structure of ferredoxin: thioredoxin reductase and thioredoxin f2 complex

Released:
DOI: 10.2210/pdb7c2b/pdb
PDB entry 7c2b coloured by chain, front view.
PDB entry 7c2b coloured by chain, side view.
PDB entry 7c2b coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for thioredoxin isoform-based fine-tuning of ferredoxin-thioredoxin reductase activity.
Juniar L, Tanaka H, Yoshida K, Hisabori T, Kurisu G
Protein Sci 29 2538-2545 (2020)
PMID: 33015914

Function and Biology Details

Reaction catalysed: 
2 reduced ferredoxin + thioredoxin disulfide = 2 oxidized ferredoxin + thioredoxin + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-185280 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ferredoxin-thioredoxin reductase catalytic chain, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9SJ89 (Residues: 32-146; Coverage: 79%)
Gene names: At2g04700, F28I8.26, FTRB, FTRC
Sequence domains: Ferredoxin thioredoxin reductase catalytic beta chain
Ferredoxin-thioredoxin reductase subunit A2, chloroplastic Chain: B
Molecule details ›
Chain: B
Length: 112 amino acids
Theoretical weight: 12.58 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8LBP6 (Residues: 73-184; Coverage: 61%)
Gene names: At5g08410, F8L15.14, FTRA2
Sequence domains: Ferredoxin thioredoxin reductase variable alpha chain
Thioredoxin F2, chloroplastic Chain: C
Molecule details ›
Chain: C
Length: 115 amino acids
Theoretical weight: 12.86 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9XFH9 (Residues: 71-185; Coverage: 62%)
Gene names: At5g16400, MQK4.13
Sequence domains: Thioredoxin

Ligands and Environments

3 bound ligands:
Ligand SF4 1 x SF4
Ligand NO3 14 x NO3
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 68.738Å b: 83.943Å c: 69.041Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.21
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Exploring the Diversity of the Thioredoxin Systems in Cyanobacteria.
Mallén-Ponce et al. (2022)
2 more

3 mentions without citation

The synaptotagmin C2B domain calcium-binding loops modulate the rate of fusion pore expansion.
Bendahmane et al. (2018)
2 more