› 7c3f

X-ray diffraction
2.4Å resolution

Crystal structure of ferredoxin: thioredoxin reductase and thioredoxin m2 complex

Released:
DOI: 10.2210/pdb7c3f/pdb
PDB entry 7c3f coloured by chain, front view.
PDB entry 7c3f coloured by chain, side view.
PDB entry 7c3f coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for thioredoxin isoform-based fine-tuning of ferredoxin-thioredoxin reductase activity.
Juniar L, Tanaka H, Yoshida K, Hisabori T, Kurisu G
Protein Sci 29 2538-2545 (2020)
PMID: 33015914

Function and Biology Details

Reaction catalysed: 
2 reduced ferredoxin + thioredoxin disulfide = 2 oxidized ferredoxin + thioredoxin + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
PDBe Complex ID:
PDB-CPX-185278 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ferredoxin-thioredoxin reductase catalytic chain, chloroplastic Chains: A, D, G, J, M, P, S, V
Molecule details ›
Chains: A, D, G, J, M, P, S, V
Length: 115 amino acids
Theoretical weight: 13 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9SJ89 (Residues: 32-146; Coverage: 79%)
Gene names: At2g04700, F28I8.26, FTRB, FTRC
Sequence domains: Ferredoxin thioredoxin reductase catalytic beta chain
Ferredoxin-thioredoxin reductase subunit A2, chloroplastic Chains: B, E, H, K, N, Q, T
Molecule details ›
Chains: B, E, H, K, N, Q, T
Length: 112 amino acids
Theoretical weight: 12.58 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8LBP6 (Residues: 73-184; Coverage: 61%)
Gene names: At5g08410, F8L15.14, FTRA2
Sequence domains: Ferredoxin thioredoxin reductase variable alpha chain
Thioredoxin M2, chloroplastic Chains: C, F, I, L, O, R, U, W
Molecule details ›
Chains: C, F, I, L, O, R, U, W
Length: 114 amino acids
Theoretical weight: 12.51 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9SEU8 (Residues: 73-186; Coverage: 61%)
Gene names: At4g03520, F9H3.15, T5L23.1, TRXM2
Sequence domains: Thioredoxin

Ligands and Environments

2 bound ligands:
Ligand SF4 7 x SF4
Ligand NA 10 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: C2
Unit cell:
a: 174.262Å b: 137.209Å c: 192.457Å
α: 90° β: 90.21° γ: 90°
R-values:
R R work R free
0.211 0.21 0.245
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Exploring the Diversity of the Thioredoxin Systems in Cyanobacteria.
Mallén-Ponce et al. (2022)
2 more

3 mentions without citation

Structural basis for thioredoxin isoform-based fine-tuning of ferredoxin-thioredoxin reductase activity.
Juniar et al. (2020)
2 more