› 7c4d

X-ray diffraction
2.03Å resolution

Marine microorganism esterase

Released:
DOI: 10.2210/pdb7c4d/pdb
PDB entry 7c4d coloured by chain, front view.
PDB entry 7c4d coloured by chain, side view.
PDB entry 7c4d coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Structural Insights into a Novel Esterase from the East Pacific Rise and Its Improved Thermostability by a Semirational Design.
Zhu C, Chen Y, Isupov MN, Littlechild JA, Sun L, Liu X, Wang Q, Gong H, Dong P, Zhang N, Wu Y
J Agric Food Chem 69 1079-1090 (2021)
PMID: 33445864

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 30.65 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2S1GUX0 (Residues: 2-261; Coverage: 97%)
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

3 bound ligands:
Ligand EDO 2 x EDO
Ligand ACT 1 x ACT
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P32
Unit cell:
a: 67Å b: 67Å c: 62Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.2 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Protein engineering of NADH pyrophosphatase for efficient biocatalytic production of reduced nicotinamide mononucleotide.
Liu et al. (2023)
1 more