› 7c6h

X-ray diffraction
1.85Å resolution

Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in an open-liganded state bound to laminaribiose

Released:
DOI: 10.2210/pdb7c6h/pdb
PDB entry 7c6h coloured by chain, front view.
PDB entry 7c6h coloured by chain, side view.
PDB entry 7c6h coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Conformational Trapping of a β-Glucosides-Binding Protein Unveils the Selective Two-Step Ligand-Binding Mechanism of ABC Importers.
Chandravanshi M, Samanta R, Kanaujia SP
J Mol Biol 432 5711-5734 (2020)
PMID: 32866452

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sugar ABC transporter, periplasmic sugar-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 422 amino acids
Theoretical weight: 45.91 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53W80 (Residues: 19-434; Coverage: 100%)
Gene name: TTHB082
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
6 bound ligands:
Ligand CL 2 x CL
Ligand ACT 1 x ACT
Ligand SO4 2 x SO4
Ligand EDO 5 x EDO
Ligand PGO 1 x PGO
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 62.51Å b: 92.25Å c: 69.78Å
α: 90° β: 112.42° γ: 90°
R-values:
R R work R free
0.17 0.168 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Deciphering Cellodextrin and Glucose Uptake in Clostridium thermocellum.
Yan et al. (2022)
1 more