› 7c83

X-ray diffraction
2Å resolution

Crystal structure of an integral membrane steroid 5-alpha-reductase PbSRD5A

Released:
DOI: 10.2210/pdb7c83/pdb
PDB entry 7c83 coloured by chain, front view.
PDB entry 7c83 coloured by chain, side view.
PDB entry 7c83 coloured by chain, top view.
Source organism: Proteobacteria bacterium
Primary publication:
Crystal structure of steroid reductase SRD5A reveals conserved steroid reduction mechanism.
Han Y, Zhuang Q, Sun B, Lv W, Wang S, Xiao Q, Pang B, Zhou Y, Wang F, Chi P, Wang Q, Li Z, Zhu L, Li F, Deng D, Chiang YC, Li Z, Ren R
Nat Commun 12 449 (2021)
PMID: 33469028

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 29.06 KDa
Source organism: Proteobacteria bacterium
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A3A0FWV3 (Residues: 1-253; Coverage: 100%)
Gene name: DCC71_03205
Sequence domains: 3-oxo-5-alpha-steroid 4-dehydrogenase

Ligands and Environments

2 bound ligands:
Ligand NDP 1 x NDP
Ligand OLC 8 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2221
Unit cell:
a: 52.192Å b: 104.266Å c: 123.046Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.193 0.233
Expression system: Spodoptera frugiperda

Quick links

Citations

13 citation in other articles

The structural basis for the phospholipid remodeling by lysophosphatidylcholine acyltransferase 3.
Zhang et al. (2021)
12 more

2 mentions without citation

Cloning, computational analysis and expression profiling of steroid 5 alpha-reductase 1 (SRD5A1) gene during reproductive phases and ovatide stimulation in endangered catfish, Clarias magur.
Agarwal et al. (2023)
1 more