› 7c91

X-ray diffraction
1.98Å resolution

Blasnase-T13A with D-asn

Released:
DOI: 10.2210/pdb7c91/pdb
Model geometry
Fit model/data
PDB entry 7c91 coloured by chain, front view.
PDB entry 7c91 coloured by chain, side view.
PDB entry 7c91 coloured by chain, top view.
Source organism: Bacillus paralicheniformis
Primary publication:
Structures of l-asparaginase from Bacillus licheniformis Reveal an Essential Residue for its Substrate Stereoselectivity.
Ran T, Jiao L, Wang W, Chen J, Chi H, Lu Z, Zhang C, Xu D, Lu F
J Agric Food Chem 69 223-231 (2021)
PMID: 33371681

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105643 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
asparaginase Chains: A, B
Molecule details ›
Chains: A, B
Length: 376 amino acids
Theoretical weight: 41.44 KDa
Source organism: Bacillus paralicheniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6I7U6Y2 (Residues: 1-322; Coverage: 100%)
Gene names: B4121_3474, PVN32_05695
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand DSG 2 x DSG
Ligand MG 5 x MG
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P41212
Unit cell:
a: 92.26Å b: 92.26Å c: 231.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.211
Expression system: Escherichia coli

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Citations

6 citation in other articles

Characterization of a Novel L-Asparaginase from Mycobacterium gordonae with Acrylamide Mitigation Potential.
Chi et al. (2021)
5 more

2 mentions without citation

Discovery of novel benzophenone integrated derivatives as anti-Alzheimer's agents targeting presenilin-1 and presenilin-2 inhibition: A computational approach.
Martiz et al. (2022)
1 more