7can

X-ray diffraction
2.94Å resolution

Structure of sybody MR17-K99Y in complex with the SARS-CoV-2 S Receptor-binding domain (RBD)

Released:
Model geometry
Fit model/data

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-276066 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
sybody MR17-K99Y Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.57 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Spike protein S1 Chain: B
Molecule details ›
Chain: B
Length: 213 amino acids
Theoretical weight: 23.75 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0DTC2 (Residues: 330-531; Coverage: 16%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P3221
Unit cell:
a: 74.191Å b: 74.191Å c: 158.404Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.214 0.267
Expression systems:
  • Escherichia coli BL21(DE3)
  • Trichoplusia ni