› 7cg6

X-ray diffraction
2Å resolution

Vigna radiata Epoxide hydrolase mutant M263Q

Released:
DOI: 10.2210/pdb7cg6/pdb
PDB entry 7cg6 coloured by chain, front view.
PDB entry 7cg6 coloured by chain, side view.
PDB entry 7cg6 coloured by chain, top view.
Source organism: Vigna radiata
Primary publication:
Reprogramming Epoxide Hydrolase to Improve Enantioconvergence in Hydrolysis of Styrene Oxide Scaffolds
Li FL, Qiu YY, Zheng YC, Chen FF, Kong XD, Xu JH, Yu HL
Adv. Synth. Catal. 362 4699-4706 (2021)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 328 amino acids
Theoretical weight: 37.52 KDa
Source organism: Vigna radiata
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0R5NGA4 (Residues: 5-318; Coverage: 99%)
Gene name: EH2
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P43212
Unit cell:
a: 70.813Å b: 70.813Å c: 127.377Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.219 0.296
Expression system: Escherichia coli BL21(DE3)

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