› 7cgo

Electron Microscopy
3.9Å resolution

Cryo-EM structure of the flagellar motor-hook complex from Salmonella

Released:
DOI: 10.2210/pdb7cgo/pdb
PDB entry 7cgo coloured by chain, front view.
PDB entry 7cgo coloured by chain, side view.
PDB entry 7cgo coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Structural basis of assembly and torque transmission of the bacterial flagellar motor.
Tan J, Zhang X, Wang X, Xu C, Chang S, Wu H, Wang T, Liang H, Gao H, Zhou Y, Zhu Y
Cell 184 2665-2679.e19 (2021)
PMID: 33882274
Related structures: EMD-30359

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 219-mer (preferred)
PDBe Complex ID:
PDB-CPX-140993 (preferred)
Entry contents:
16 distinct polypeptide molecules
Macromolecules (16 distinct):
Flagellar basal-body rod protein FlgG Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 260 amino acids
Theoretical weight: 27.78 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1J3 (Residues: 1-260; Coverage: 100%)
Gene names: STM1179, fla FVII, flaL, flgG
Sequence domains:
Flagellar basal-body rod protein FlgF Chains: a, b, c, d, e
Molecule details ›
Chains: a, b, c, d, e
Length: 251 amino acids
Theoretical weight: 26.12 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P16323 (Residues: 1-251; Coverage: 100%)
Gene names: STM1178, fla FVI, flaX, flgF
Sequence domains:
Flagellar MS ring L2 Chains: 0, 1, 2, 3, 4
Molecule details ›
Chains: 0, 1, 2, 3, 4
Length: 15 amino acids
Theoretical weight: 1.29 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Flagellar M-ring protein Chains: 5, 6, 7, 8, 9
Molecule details ›
Chains: 5, 6, 7, 8, 9
Length: 21 amino acids
Theoretical weight: 2 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P15928 (Residues: 311-331; Coverage: 4%)
Gene names: STM1969, fla AII.1, fla BI, fliF
Flagellar basal-body rod protein FlgC Chains: f, g, h, i, j, p
Molecule details ›
Chains: f, g, h, i, j, p
Length: 134 amino acids
Theoretical weight: 13.99 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1I7 (Residues: 1-134; Coverage: 100%)
Gene names: STM1175, fla FIII, flaW, flgC
Sequence domains:
Flagellar basal body rod protein FlgB Chains: k, l, m, n, o
Molecule details ›
Chains: k, l, m, n, o
Length: 138 amino acids
Theoretical weight: 15.15 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P16437 (Residues: 1-138; Coverage: 100%)
Gene names: STM1174, fla FII, flbA, flgB
Sequence domains: Flagella basal body rod protein
Flagellar hook-basal body complex protein FliE Chains: q, r, s, t, u, v
Molecule details ›
Chains: q, r, s, t, u, v
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P26462 (Residues: 1-104; Coverage: 100%)
Gene names: STM1968, fla AI, flaN, fliE
Sequence domains: Flagellar hook-basal body complex protein FliE
Flagellar hook protein FlgE Chains: DA, DB, DC, DD, DE, DF, DG, DH, DI, DJ, DK, DL, DM, DN, DO, DP, DQ, DR, DS, DT, DU, DV, DW, DX, DY, DZ, EA, EB, EC, ED, EE, EF, EG
Molecule details ›
Chains: DA, DB, DC, DD, DE, DF, DG, DH, DI, DJ, DK, DL, DM, DN, DO, DP, DQ, DR, DS, DT, DU, DV, DW, DX, DY, DZ, EA, EB, EC, ED, EE, EF, EG
Length: 403 amino acids
Theoretical weight: 42.23 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1J1 (Residues: 1-403; Coverage: 100%)
Gene names: STM1177, fla FV, flaK, flgE
Sequence domains:
Flagellar biosynthetic protein FliR Chain: CE
Molecule details ›
Chain: CE
Length: 264 amino acids
Theoretical weight: 28.94 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P54702 (Residues: 1-264; Coverage: 100%)
Gene names: STM1981, flaP, fliR
Sequence domains: Bacterial export proteins, family 1
Flagellar biosynthetic protein FliQ Chains: CA, CB, CC, CD
Molecule details ›
Chains: CA, CB, CC, CD
Length: 89 amino acids
Theoretical weight: 9.61 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1L5 (Residues: 1-89; Coverage: 100%)
Gene names: STM1980, flaQ, fliQ
Sequence domains: Bacterial export proteins, family 3
Flagellar biosynthetic protein FliP Chains: CF, w, x, y, z
Molecule details ›
Chains: CF, w, x, y, z
Length: 245 amino acids
Theoretical weight: 26.8 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P54700 (Residues: 1-245; Coverage: 100%)
Gene names: STM1979, flaR, fliP
Sequence domains: FliP family
Flagellar M-ring protein Chains: Ca, Cb, Cc, Cd, Ce, Cf, Cg, Ch, Ci, Cj, Ck, Cl, Cm, Cn, Co, Cp, Cq, Cr, Cs, Ct, Cu, Cv, Cw, Cx, Cy, Cz, Da, Db, Dc, Dd, De, Df, Dg, Dh, Di, Dj, Dk, Dl, Dm, Dn, Do, Dp, Dq, Dr, Ds, Dt, Du, Dv, Dw, Ea, Eb, Ec, Ed, Ee, Ef, Eg, Eh
Molecule details ›
Chains: Ca, Cb, Cc, Cd, Ce, Cf, Cg, Ch, Ci, Cj, Ck, Cl, Cm, Cn, Co, Cp, Cq, Cr, Cs, Ct, Cu, Cv, Cw, Cx, Cy, Cz, Da, Db, Dc, Dd, De, Df, Dg, Dh, Di, Dj, Dk, Dl, Dm, Dn, Do, Dp, Dq, Dr, Ds, Dt, Du, Dv, Dw, Ea, Eb, Ec, Ed, Ee, Ef, Eg, Eh
Length: 560 amino acids
Theoretical weight: 61.3 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P15928 (Residues: 1-560; Coverage: 100%)
Gene names: STM1969, fla AII.1, fla BI, fliF
Sequence domains:
FlgB-Dc loop Chains: GA, GB, GC, GD, GE
Molecule details ›
Chains: GA, GB, GC, GD, GE
Length: 12 amino acids
Theoretical weight: 1.04 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
FliE helix 1 Chains: GF, GG, GH, GI, GJ, GK
Molecule details ›
Chains: GF, GG, GH, GI, GJ, GK
Length: 18 amino acids
Theoretical weight: 1.55 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Flagellar L-ring protein Chains: AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR, AS, AT, AU, AV, AW, AX, AY, AZ
Molecule details ›
Chains: AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR, AS, AT, AU, AV, AW, AX, AY, AZ
Length: 232 amino acids
Theoretical weight: 24.97 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1N8 (Residues: 1-232; Coverage: 100%)
Gene names: STM1180, fla FVIII, flaY, flgH
Sequence domains: Flagellar L-ring protein
Flagellar P-ring protein Chains: BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY, BZ
Molecule details ›
Chains: BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY, BZ
Length: 365 amino acids
Theoretical weight: 38.19 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P15930 (Residues: 1-365; Coverage: 100%)
Gene names: STM1181, fla FIX, flaM, flgI
Sequence domains: Flagellar P-ring protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand P1L 26 x P1L

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-30359

Quick links

Citations

7 review citations

Bacterial motility: machinery and mechanisms.
Wadhwa et al. (2022)
6 more

4 mentions without citation

3D cryo-EM imaging of bacterial flagella: Novel structural and mechanistic insights into cell motility.
Mondino et al. (2022)
3 more