› 7ci0

X-ray diffraction
1.7Å resolution

Microbial Hormone-sensitive lipase E53 mutant S162A

Released:
DOI: 10.2210/pdb7ci0/pdb
PDB entry 7ci0 coloured by chain, front view.
PDB entry 7ci0 coloured by chain, side view.
PDB entry 7ci0 coloured by chain, top view.
Source organism: Erythrobacter longus
Primary publication:
Mechanism and Structural Insights Into a Novel Esterase, E53, Isolated From Erythrobacter longus.
Ding Y, Nie L, Yang XC, Li Y, Huo YY, Li Z, Gao Y, Cui HL, Li J, Xu XW
Front Microbiol 12 798194 (2021)
PMID: 35069500

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 314 amino acids
Theoretical weight: 33.14 KDa
Source organism: Erythrobacter longus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A074MDU6 (Residues: 1-314; Coverage: 100%)
Gene name: EH31_02760
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

11 bound ligands:
Ligand NPO 11 x NPO
Ligand 6NA 3 x 6NA
Ligand PEG 2 x PEG
Ligand EDO 10 x EDO
Ligand DMS 14 x DMS
Ligand DIO 6 x DIO
Ligand GOL 9 x GOL
Ligand SO4 5 x SO4
Ligand PGE 2 x PGE
Ligand NA 1 x NA
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Unit cell:
a: 70.397Å b: 129.771Å c: 220.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.191
Expression system: Escherichia coli K-12

Quick links

Citations

1 citations in other articles

The Mobility of the Cap Domain Is Essential for the Substrate Promiscuity of a Family IV Esterase from Sorghum Rhizosphere Microbiome.
Distaso et al. (2023)

1 mention without citation

Mechanism and Structural Insights Into a Novel Esterase, E53, Isolated From Erythrobacter longus.
Ding et al. (2021)