› 7ckq

Electron Microscopy
4.4Å resolution

The cryo-EM structure of B. subtilis BmrR transcription activation complex

Released:
DOI: 10.2210/pdb7ckq/pdb
PDB entry 7ckq coloured by chain, front view.
PDB entry 7ckq coloured by chain, side view.
PDB entry 7ckq coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
The bacterial multidrug resistance regulator BmrR distorts promoter DNA to activate transcription.
Fang C, Li L, Zhao Y, Wu X, Philips SJ, You L, Zhong M, Shi X, O'Halloran TV, Li Q, Zhang Y
Nat Commun 11 6284 (2020)
PMID: 33293519
Related structures: EMD-30390

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-121154 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (9 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 34.84 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: P20429 (Residues: 1-314; Coverage: 100%)
Gene names: BSU01430, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1193 amino acids
Theoretical weight: 133.85 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: P37870 (Residues: 1-1193; Coverage: 100%)
Gene names: BSU01070, crsE, rfm, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1199 amino acids
Theoretical weight: 134.44 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: P37871 (Residues: 1-1199; Coverage: 100%)
Gene names: BSU01080, lpm, rpoC, std
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 67 amino acids
Theoretical weight: 7.77 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: O35011 (Residues: 1-67; Coverage: 100%)
Gene names: BSU15690, rpoZ, yloH
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor SigA Chain: F
Molecule details ›
Chain: F
Length: 371 amino acids
Theoretical weight: 43.01 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: P06224 (Residues: 1-371; Coverage: 100%)
Gene names: BSU25200, rpoD, sigA
Sequence domains:
Multidrug-efflux transporter 1 regulator Chains: G, I
Molecule details ›
Chains: G, I
Length: 282 amino acids
Theoretical weight: 32.95 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39075 (Residues: 1-278; Coverage: 100%)
Gene names: BSU24020, bmr1R, bmrR
Sequence domains:
DNA-directed RNA polymerase subunit epsilon Chain: H
Molecule details ›
Chain: H
Length: 69 amino acids
Theoretical weight: 8.26 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: O31718 (Residues: 1-69; Coverage: 100%)
Gene names: BSU14540, rpoY, ykzG
Sequence domains: RNA polymerase epsilon subunit
DNA (50-MER) Chain: 1
Molecule details ›
Chain: 1
Length: 50 nucleotides
Theoretical weight: 15.49 KDa
DNA (50-MER) Chain: 2
Molecule details ›
Chain: 2
Length: 50 nucleotides
Theoretical weight: 15.49 KDa

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ZN 2 x ZN
Ligand P4P 2 x P4P
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-30390
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Advances in Understanding of the Copper Homeostasis in Pseudomonas aeruginosa.
Hofmann et al. (2021)
1 more

11 mentions without citation

Bacterial MerR family transcription regulators: activationby distortion.
Fang et al. (2022)
10 more