› 7cn2

Electron Microscopy
3.43Å resolution

Subparticle refinement of human papillomavirus type 16 pesudovirus in complex with H16.001 Fab

Released:
DOI: 10.2210/pdb7cn2/pdb
PDB entry 7cn2 coloured by chain, front view.
PDB entry 7cn2 coloured by chain, side view.
PDB entry 7cn2 coloured by chain, top view.
Source organisms:
Primary publication:
Structural characterization of a neutralizing mAb H16.001, a potent candidate for a common potency assay for various HPV16 VLPs.
Huang W, He M, Ning T, Nie J, Zhang F, Zheng Q, Zhang R, Xu Y, Gu Y, Li S, Wang Y
NPJ Vaccines 5 89 (2020)
PMID: 33042588
Related structures: EMD-30414

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208884 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
The light chain variable region of H16.001 Fab fragment Chains: L, g, i, j, k, m
Molecule details ›
Chains: L, g, i, j, k, m
Length: 110 amino acids
Theoretical weight: 11.51 KDa
Source organism: Oryctolagus cuniculus
The heavy chain variable region of H16.001 Fab fragment Chains: G, H, I, J, K, M
Molecule details ›
Chains: G, H, I, J, K, M
Length: 120 amino acids
Theoretical weight: 13.21 KDa
Source organism: Oryctolagus cuniculus
Major capsid protein L1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 505 amino acids
Theoretical weight: 56.34 KDa
Source organism: Human papillomavirus 16
Expression system: Homo sapiens
UniProt:
  • Canonical: P03101 (Residues: 1-505; Coverage: 100%)
Gene name: L1
Sequence domains: L1 (late) protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.43Å
Relevant EMDB volumes: EMD-30414
Expression system: Homo sapiens

Quick links

Citations

1 review citation

Development of functionally relevant potency assays for monovalent and multivalent vaccines delivered by evolving technologies.
Sanyal G. (2022)
2 other articles

1 mention without citation

B-cell epitope discovery: The first protein flexibility-based algorithm-Zika virus conserved epitope demonstration.
Biner et al. (2023)