› 7cne

X-ray diffraction
2Å resolution

Crystal Structure of Sphingomyelinase C from Streptomyces griseocarneus

Released:
DOI: 10.2210/pdb7cne/pdb
PDB entry 7cne coloured by chain, front view.
PDB entry 7cne coloured by chain, side view.
PDB entry 7cne coloured by chain, top view.
Source organism: Streptomyces griseocarneus
Primary publication:
Structural basis for the high thermal stability and optimum pH of sphingomyelinase C from Streptomyces griseocarneus.
Fujisawa I, Hamana H, Tomita Y, Matsumoto Y, Murayama K, Sugimori D
J Biosci Bioeng 131 8-12 (2021)
PMID: 33023861

Function and Biology Details

Reaction catalysed: 
Sphingomyelin + H(2)O = N-acylsphingosine + phosphocholine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease/exonuclease/phosphatase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 32.22 KDa
Source organism: Streptomyces griseocarneus
Expression system: Streptomyces lividans
UniProt:
  • Canonical: A6P7M9 (Residues: 43-339; Coverage: 100%)
Gene name: smc
Sequence domains: Endonuclease/Exonuclease/phosphatase family

Ligands and Environments

2 bound ligands:
Ligand EPE 1 x EPE
Ligand SIN 1 x SIN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P41212
Unit cell:
a: 66.091Å b: 66.091Å c: 142.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.163 0.201
Expression system: Streptomyces lividans

Quick links

Citations

1 mention without citation

Metagenomic characterization of sphingomyelinase C in the microbiome of humans and environments.
Jeon et al. (2022)