› 7csi

X-ray diffraction
1.89Å resolution

Aminoglycoside 2'-N-acetyltransferase from Mycolicibacterium smegmatis-Complex with Coenzyme A and Sisomicin

Released:
DOI: 10.2210/pdb7csi/pdb
Model geometry
Fit model/data
PDB entry 7csi coloured by chain, front view.
PDB entry 7csi coloured by chain, side view.
PDB entry 7csi coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structural and biochemical analyses of an aminoglycoside 2'-N-acetyltransferase from Mycolicibacterium smegmatis.
Jeong CS, Hwang J, Do H, Cha SS, Oh TJ, Kim HJ, Park HH, Lee JH
OpenAccess logo Sci Rep 10 21503 (2020)
PMID: 33299080

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside 2'-N-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 23.2 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: P94968 (Residues: 1-210; Coverage: 100%)
Gene names: MSMEG_0434, MSMEI_0423, aac
Sequence domains: Acetyltransferase (GNAT) family

Ligands and Environments

2 bound ligands:
Ligand SIS 2 x SIS
Ligand COA 2 x COA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21212
Unit cell:
a: 77.828Å b: 93.457Å c: 61.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.215
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Identification and characterization of a novel GNAT superfamily Nα -acetyltransferase from Salinicoccus halodurans H3B36.
Ma et al. (2022)
1 more

1 mention without citation

Structural and biochemical analyses of an aminoglycoside 2'-N-acetyltransferase from Mycolicibacterium smegmatis.
Jeong et al. (2020)