› 7csl

X-ray diffraction
2Å resolution

Crystal structure of the archaeal EF1A-EF1B complex

Released:
DOI: 10.2210/pdb7csl/pdb
Model geometry
Fit model/data
PDB entry 7csl coloured by chain, front view.
PDB entry 7csl coloured by chain, side view.
PDB entry 7csl coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Structural insights into the Switching Off of the Interaction between the Archaeal Ribosomal Stalk and aEF1A by Nucleotide Exchange Factor aEF1B.
Suzuki T, Ito K, Miyoshi T, Murakami R, Uchiumi T
J Mol Biol 433 167046 (2021)
PMID: 33971210

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129814 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Elongation factor 1-alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 434 amino acids
Theoretical weight: 48.43 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59153 (Residues: 1-428; Coverage: 100%)
Gene names: PH1484, PHCC033, tuf
Sequence domains:
Elongation factor 1-beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 91 amino acids
Theoretical weight: 10.36 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: P58748 (Residues: 1-91; Coverage: 100%)
Gene names: PH0026.1, ef1b
Sequence domains: EF-1 guanine nucleotide exchange domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P1
Unit cell:
a: 55.149Å b: 70.194Å c: 77.822Å
α: 112.9° β: 91.77° γ: 89.98°
R-values:
R R work R free
0.227 0.225 0.275
Expression system: Escherichia coli

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