› 7cy0

X-ray diffraction
1.32Å resolution

Crystal structure of S185H mutant PET hydrolase from Ideonella sakaiensis

Released:
DOI: 10.2210/pdb7cy0/pdb
PDB entry 7cy0 coloured by chain, front view.
PDB entry 7cy0 coloured by chain, side view.
PDB entry 7cy0 coloured by chain, top view.
Source organism: Ideonella sakaiensis
Primary publication:
General features to enhance enzymatic activity of poly(ethylene terephthalate) hydrolysis.
Che CC, Han X, Li X, Jiang P, Niu D, Ma L, Liu W, Li S, Qu Y, Hu H, Min J, Yang Y, Zhang L, Zeng W, Huang JW, Dai L, Guo RT
Nat Catal 4 425-430 (2021)

Function and Biology Details

Reaction catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(ethylene terephthalate) hydrolase Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 27.29 KDa
Source organism: Ideonella sakaiensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0K8P6T7 (Residues: 30-290; Coverage: 99%)
Gene name: ISF6_4831
Sequence domains: Dienelactone hydrolase family

Ligands and Environments

1 bound ligand:
Ligand ACY 3 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P212121
Unit cell:
a: 50.925Å b: 51.806Å c: 84.287Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.166
Expression system: Escherichia coli BL21(DE3)

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