› 7cz0

X-ray diffraction
2.77Å resolution

Crystal structure of a thermostable green fluorescent protein (TGP) with a synthetic nanobody (Sb92)

Released:
DOI: 10.2210/pdb7cz0/pdb
PDB entry 7cz0 coloured by chain, front view.
PDB entry 7cz0 coloured by chain, side view.
PDB entry 7cz0 coloured by chain, top view.
Source organisms:
Entry authors: Cai H, Yao H, Li T, Hutter C, Tang Y, Li Y, Seeger M, Li D

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-211789 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thermostable green fluorescent protein (TGP) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 232 amino acids
Theoretical weight: 26.7 KDa
Source organism: Galaxea fascicularis
Expression system: Escherichia coli BL21(DE3)
Synthetic nanobody (Sybody) 92 recognizing the thermostable green fluorescent protein (TGP) Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 144 amino acids
Theoretical weight: 15.81 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

4 bound ligands:
Ligand GOL 11 x GOL
Ligand CAD 1 x CAD
Ligand ACT 2 x ACT
Ligand CAC 1 x CAC
1 modified residue:
Ligand CRQ 4 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21212
Unit cell:
a: 95.245Å b: 130.154Å c: 171.453Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.241
Expression system: Escherichia coli BL21(DE3)

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