› 7d08

Electron Microscopy
4Å resolution

Acinetobacter MlaFEDB complex in ATP-bound Vtrans1 conformation

Released:
DOI: 10.2210/pdb7d08/pdb
PDB entry 7d08 coloured by chain, front view.
PDB entry 7d08 coloured by chain, side view.
PDB entry 7d08 coloured by chain, top view.
Source organism: Acinetobacter baumannii
Primary publication:
Cryo-EM structures of Acinetobacter baumannii glycerophospholipid transporter.
Zhang Y, Fan Q, Chi X, Zhou Q, Li Y
Cell Discov 6 86 (2020)
PMID: 33298869
Related structures: EMD-30526

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-100586 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Intermembrane phospholipid transport system permease protein MlaE Chains: A, D
Molecule details ›
Chains: A, D
Length: 258 amino acids
Theoretical weight: 27.32 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: V5V9F4 (Residues: 1-258; Coverage: 100%)
Gene names: A7M90_14775, ABR2091_3298, APD17_09690, AUO97_04515, B9X95_19830, CAS83_02490, CV954_002080, EA706_02625, EA722_02115, F4T85_12835, FJU42_03310, G3N53_10430, GSE42_18090, ITE13_05885, JHZ39_001261, MKP18_001965, P9867_02730, mlaE
Sequence domains: Permease MlaE
ABC transporter domain-containing protein Chains: B, E
Molecule details ›
Chains: B, E
Length: 273 amino acids
Theoretical weight: 30.07 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A086HZU3 (Residues: 1-272; Coverage: 100%)
Gene names: A7M90_14770, ABR2091_3299, APD17_09695, AUO97_04520, B9W25_03290, CBE85_06065, CV954_002075, DOL94_03235, G3N53_10435, GSE42_18095, IAG11_07270, IMO23_16130, ITE13_05880, JHZ39_001260, MKP18_001964, SAMEA104305318_02181, SAMEA4394745_00274, ttg2A, yrbF
Sequence domains: ABC transporter
STAS domain-containing protein Chains: C, F
Molecule details ›
Chains: C, F
Length: 103 amino acids
Theoretical weight: 11.91 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: V5V9K5 (Residues: 2-95; Coverage: 99%)
Gene names: A7M90_14790, ABR2091_3295, ABUW_0387, APD17_09675, AUO97_04500, B9W25_03310, B9X95_19845, CAS83_02475, CBE85_06045, CV954_002095, DOL94_03255, EA686_18495, EA706_02640, EA722_02130, F2P40_01390, F4T85_12820, FJU42_03325, G3N53_10415, GNY86_20590, GSE42_18075, IAG11_07250, IMO23_16110, ITE13_05900, MKP18_001968, P9867_02745, SAMEA4394745_00270, mlaB
Sequence domains: STAS domain
Mce/MlaD domain-containing protein Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 222 amino acids
Theoretical weight: 23.67 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: V5V921 (Residues: 1-222; Coverage: 98%)
Gene names: A7M90_14780, ABR2091_3297, ABUW_0385, APD17_09685, AUO97_04510, B9W25_03300, B9X95_19835, CAS83_02485, CBE85_06055, CV954_002085, DOL94_03245, EA706_02630, EA722_02120, F2P40_01380, F4T85_12830, G3N53_10425, GNY86_20580, GSE42_18085, IAG11_07260, IMO23_16120, ITE13_05890, JHZ39_001262, P9867_02735, SAMEA104305318_02183, SAMEA4394745_00272, mlaD, ttg2C
Sequence domains: MlaD protein

Ligands and Environments

1 bound ligand:
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-30526
Expression system: Escherichia coli K-12

Quick links

Citations

3 review citations

ABC Transporters in Bacterial Nanomachineries.
Bilsing et al. (2023)
2 more

1 mention without citation

Structure and mechanism of the bacterial lipid ABC transporter, MlaFEDB.
Ekiert et al. (2022)