› 7d3f

Electron Microscopy
2.6Å resolution

Cryo-EM structure of human DUOX1-DUOXA1 in high-calcium state

Released:
DOI: 10.2210/pdb7d3f/pdb
PDB entry 7d3f coloured by chain, front view.
PDB entry 7d3f coloured by chain, side view.
PDB entry 7d3f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of human dual oxidase 1 complex in low-calcium and high-calcium states.
Wu JX, Liu R, Song K, Chen L
OpenAccess logo Nat Commun 12 155 (2021)
PMID: 33420071
Related structures: EMD-30556

Function and Biology Details

Reaction catalysed: 
NAD(P)H + O(2) = NAD(P)(+) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172879 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Dual oxidase 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 1551 amino acids
Theoretical weight: 177.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NRD9 (Residues: 1-1551; Coverage: 100%)
Gene names: DUOX, DUOX1, LNOX1, THOX1
Sequence domains:
Dual oxidase maturation factor 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 483 amino acids
Theoretical weight: 53.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q1HG43 (Residues: 1-330; Coverage: 96%)
  • Best match: Q1HG43-2 (Residues: 1-483)
Gene names: DUOXA1, NIP, NUMBIP
Sequence domains: Dual oxidase maturation factor

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM

Cofactor: Ligand FAD 2 x FAD

Cofactor: Ligand NDP 2 x NDP
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand CA 4 x CA
Ligand NAG 10 x NAG
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.6Å
Relevant EMDB volumes: EMD-30556
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Role of Mitophagy in Regulating Intestinal Oxidative Damage.
Wen et al. (2023)
2 more

7 mentions without citation

Architecture of the NADPH oxidase family of enzymes.
Ogboo et al. (2022)
6 more