› 7d5q

X-ray diffraction
3.6Å resolution

Structure of NorC transporter (K398A mutant) in an outward-open conformation in complex with a single-chain Indian camelid antibody

Released:
DOI: 10.2210/pdb7d5q/pdb
PDB entry 7d5q coloured by chain, front view.
PDB entry 7d5q coloured by chain, side view.
PDB entry 7d5q coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of inhibition of a transporter from Staphylococcus aureus, NorC, through a single-domain camelid antibody.
Kumar S, Athreya A, Gulati A, Nair RM, Mahendran I, Ranjan R, Penmatsa A
Commun Biol 4 836 (2021)
PMID: 34226658

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207279 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Quinolone resistance protein NorB Chains: A, B
Molecule details ›
Chains: A, B
Length: 479 amino acids
Theoretical weight: 50.86 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2WZS4 (Residues: 1-462; Coverage: 100%)
Gene name: SACOL0086
Sequence domains: Sugar (and other) transporter
ICab Chains: C, D
Molecule details ›
Chains: C, D
Length: 131 amino acids
Theoretical weight: 14.26 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 70.071Å b: 139.148Å c: 115.555Å
α: 90° β: 105.66° γ: 90°
R-values:
R R work R free
0.308 0.308 0.315
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Efflux Pump Mediated Antimicrobial Resistance by Staphylococci in Health-Related Environments: Challenges and the Quest for Inhibition.
Dashtbani-Roozbehani et al. (2021)
2 more