› 7d78

X-ray diffraction
1.97Å resolution

The structure of thioesterase DcsB

Released:
DOI: 10.2210/pdb7d78/pdb
PDB entry 7d78 coloured by chain, front view.
PDB entry 7d78 coloured by chain, side view.
PDB entry 7d78 coloured by chain, top view.
Source organism: Beauveria bassiana ARSEF 2860
Primary publication:
A Polyketide Cyclase That Forms Medium-Ring Lactones.
Gao DW, Jamieson CS, Wang G, Yan Y, Zhou J, Houk KN, Tang Y
J Am Chem Soc 143 80-84 (2021)
PMID: 33351624

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 322 amino acids
Theoretical weight: 35.17 KDa
Source organism: Beauveria bassiana ARSEF 2860
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: J4WAT9 (Residues: 17-318; Coverage: 95%)
Gene name: BBA_03809
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand GOL 3 x GOL
Ligand CL 2 x CL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2
Unit cell:
a: 178.707Å b: 47.569Å c: 71.695Å
α: 90° β: 93.187° γ: 90°
R-values:
R R work R free
0.148 0.146 0.183
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Total Heterologous Biosynthesis of Fungal Natural Products in Aspergillus nidulans.
Chiang et al. (2022)
1 more

1 mention without citation

A Polyketide Cyclase That Forms Medium-Ring Lactones.
Gao et al. (2021)