› 7d79

X-ray diffraction
2.1Å resolution

The structure of DcsB complex with its substrate analogue

Released:
DOI: 10.2210/pdb7d79/pdb
PDB entry 7d79 coloured by chain, front view.
PDB entry 7d79 coloured by chain, side view.
PDB entry 7d79 coloured by chain, top view.
Source organism: Beauveria bassiana ARSEF 2860
Primary publication:
A Polyketide Cyclase That Forms Medium-Ring Lactones.
Gao DW, Jamieson CS, Wang G, Yan Y, Zhou J, Houk KN, Tang Y
J Am Chem Soc 143 80-84 (2021)
PMID: 33351624

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 322 amino acids
Theoretical weight: 35.17 KDa
Source organism: Beauveria bassiana ARSEF 2860
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: J4WAT9 (Residues: 17-318; Coverage: 95%)
Gene name: BBA_03809
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

6 bound ligands:
Ligand CL 4 x CL
Ligand EDO 6 x EDO
Ligand GY0 1 x GY0
Ligand GOL 2 x GOL
Ligand GYF 1 x GYF
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 47.263Å b: 59.011Å c: 113.99Å
α: 84.846° β: 78.552° γ: 84.008°
R-values:
R R work R free
0.185 0.182 0.227
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Total Heterologous Biosynthesis of Fungal Natural Products in Aspergillus nidulans.
Chiang et al. (2022)
1 more

2 mentions without citation

A Polyketide Cyclase That Forms Medium-Ring Lactones.
Gao et al. (2021)
1 more