› 7d9c

X-ray diffraction
1.36Å resolution

Alpha-glucosidase from Weissella cibaria BBK-1 bound with maltose

Released:
DOI: 10.2210/pdb7d9c/pdb
PDB entry 7d9c coloured by chain, front view.
PDB entry 7d9c coloured by chain, side view.
PDB entry 7d9c coloured by chain, top view.
Source organism: Weissella confusa
Primary publication:
A GH13 α-glucosidase from Weissella cibaria uncommonly acts on short-chain maltooligosaccharides.
Wangpaiboon K, Laohawuttichai P, Kim SY, Mori T, Nakapong S, Pichyangkura R, Pongsawasdi P, Hakoshima T, Krusong K
Acta Crystallogr D Struct Biol 77 1064-1076 (2021)
PMID: 34342279

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105103 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-glycosidase Chain: A
Molecule details ›
Chain: A
Length: 589 amino acids
Theoretical weight: 68.4 KDa
Source organism: Weissella confusa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A482PRE5 (Residues: 2-588; Coverage: 100%)
Gene name: C6P08_07940
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
5 bound ligands:
Ligand CA 1 x CA
Ligand SO4 6 x SO4
Ligand MES 2 x MES
Ligand GLC 1 x GLC
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P4122
Unit cell:
a: 115.636Å b: 115.636Å c: 106.163Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.14 0.153
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Structure and Function Insight of the α-Glucosidase QsGH13 From Qipengyuania seohaensis sp. SW-135.
Zhai et al. (2022)
4 more