› 7daj

X-ray diffraction
2.3Å resolution

The crystal structure of serotonin N-acetyltransferase in complex with acetyl-CoA from Oryza Sativa

Released:
DOI: 10.2210/pdb7daj/pdb
PDB entry 7daj coloured by chain, front view.
PDB entry 7daj coloured by chain, side view.
PDB entry 7daj coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Structural and Molecular Dynamics Analysis of Plant Serotonin N-Acetyltransferase Reveal an Acid/Base-Assisted Catalysis in Melatonin Biosynthesis.
Liao L, Zhou Y, Xu Y, Zhang Y, Liu X, Liu B, Chen X, Guo Y, Zeng Z, Zhao Y
Angew Chem Int Ed Engl 60 12020-12026 (2021)
PMID: 33682300

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177347 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serotonin N-acetyltransferase 1, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 18.51 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5KQI6 (Residues: 91-254; Coverage: 65%)
Gene names: GNAT5, LOC_Os05g40260, NSI, OSJNBa0095J22.4, Os05g0481000, OsJ_018182, OsJ_18949, SNAT, SNAT1
Sequence domains: Acetyltransferase (GNAT) family

Ligands and Environments


Cofactor: Ligand ACO 2 x ACO
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 66.838Å b: 68.188Å c: 77.985Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.2 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Melatonin-Mediated Abiotic Stress Tolerance in Plants.
Zeng et al. (2022)
2 more