› 7dcs

X-ray diffraction
2.4Å resolution

Crystal structure of HSF1 DNA-binding domain in complex with 3-site HSE DNA (23 bp)

Released:
DOI: 10.2210/pdb7dcs/pdb
PDB entry 7dcs coloured by chain, front view.
PDB entry 7dcs coloured by chain, side view.
PDB entry 7dcs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of heat shock factor trimers bound to DNA.
Feng N, Feng H, Wang S, Punekar AS, Ladenstein R, Wang DC, Zhang Q, Ding J, Liu W
iScience 24 102951 (2021)
PMID: 34458700

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121208 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Heat shock factor protein 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 113 amino acids
Theoretical weight: 13.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q00613 (Residues: 15-120; Coverage: 20%)
Gene names: HSF1, HSTF1
Sequence domains: HSF-type DNA-binding
DNA (5'-D(*TP*GP*GP*CP*GP*TP*TP*CP*TP*AP*GP*AP*AP*TP*AP*TP*TP*CP*GP*CP*GP*GP*A)-3') Chains: G, I
Molecule details ›
Chains: G, I
Length: 23 nucleotides
Theoretical weight: 7.11 KDa
Source organism: Homo sapiens
Expression system: DNA satellites
DNA (5'-D(*AP*TP*CP*CP*GP*CP*GP*AP*AP*TP*AP*TP*TP*CP*TP*AP*GP*AP*AP*CP*GP*CP*C)-3') Chains: H, J
Molecule details ›
Chains: H, J
Length: 23 nucleotides
Theoretical weight: 7.01 KDa
Source organism: Homo sapiens
Expression system: DNA satellites

Ligands and Environments

1 bound ligand:
Ligand NA 5 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 86.72Å b: 93.72Å c: 142.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.225
Expression systems:
  • Escherichia coli K-12
  • DNA satellites

Quick links

Citations

1 review citation

HIF1, HSF1, and NRF2: Oxidant-Responsive Trio Raising Cellular Defenses and Engaging Immune System.
Cyran et al. (2022)
5 other articles

1 mention without citation

Structures of heat shock factor trimers bound to DNA.
Feng et al. (2021)