› 7de0

X-ray diffraction
2.4Å resolution

Crystal structure of FtmOx1 Y224F mutation

Released:
DOI: 10.2210/pdb7de0/pdb
PDB entry 7de0 coloured by chain, front view.
PDB entry 7de0 coloured by chain, side view.
PDB entry 7de0 coloured by chain, top view.
Source organism: Aspergillus fumigatus AF210
Primary publication:
Structure-based Insights into Mechanism of Endoperoxidase FtmOx1 Catalyzed Reactions
Wang F, Gao YQ, Wang CX, Lan WX, Gan JH, Cao CY
Catal Sci Technol (2023)

Function and Biology Details

Reaction catalysed: 
Fumitremorgin B + 2-oxoglutarate + 2 O(2) + AH(2) = verruculogen + succinate + CO(2) + H(2)O + A
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Verruculogen synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 299 amino acids
Theoretical weight: 33.76 KDa
Source organism: Aspergillus fumigatus AF210
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WAW9 (Residues: 1-291; Coverage: 100%)
Gene names: AFUA_8G00230, ftmF, ftmOx1
Sequence domains: Phytanoyl-CoA dioxygenase (PhyH)

Ligands and Environments

1 bound ligand:
Ligand FE 4 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 45.436Å b: 136.74Å c: 180.628Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.263
Expression system: Escherichia coli

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