› 7dfo

X-ray diffraction
2Å resolution

Crystal structure of glycoside hydrolase family 11 beta-xylanase from Streptomyces olivaceoviridis E-86 in complex with 4-O-methyl-alpha-D-glucuronopyranosyl xylotetraose

Released:
DOI: 10.2210/pdb7dfo/pdb
PDB entry 7dfo coloured by chain, front view.
PDB entry 7dfo coloured by chain, side view.
PDB entry 7dfo coloured by chain, top view.
Source organism: Streptomyces olivaceoviridis
Primary publication:
Structure-based substrate specificity analysis of GH11 xylanase from Streptomyces olivaceoviridis E-86.
Fujimoto Z, Kishine N, Teramoto K, Tsutsui S, Kaneko S
Appl Microbiol Biotechnol 105 1943-1952 (2021)
PMID: 33564921

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Endo-1,4-beta-xylanase Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 205 amino acids
Theoretical weight: 22.54 KDa
Source organism: Streptomyces olivaceoviridis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A7G1MBT0 (Residues: 41-231; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 11

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
Carbohydrate polymer : NEW Components: XYP, GCV
Carbohydrate polymer
Carbohydrate polymer : NEW Components: XYP, GCV
Carbohydrate polymer
Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
2 bound ligands:
Ligand CL 43 x CL
Ligand NA 13 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P32
Unit cell:
a: 142.709Å b: 142.709Å c: 72.454Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.165 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Acetylated Xylan Degradation by Glycoside Hydrolase Family 10 and 11 Xylanases from the White-rot Fungus Phanerochaete chrysosporium.
Kojima et al. (2022)
2 more

1 mention without citation

Structural and biochemical analysis reveals how ferulic acid improves catalytic efficiency of Humicola grisea xylanase.
Oliveira et al. (2022)