› 7dq9

X-ray diffraction
1.7Å resolution

Crystal structure of a type-A feruloyl esterase from gut Alistipes shahii

Released:
DOI: 10.2210/pdb7dq9/pdb
PDB entry 7dq9 coloured by chain, front view.
PDB entry 7dq9 coloured by chain, side view.
PDB entry 7dq9 coloured by chain, top view.
Source organism: Alistipes shahii WAL 8301
Primary publication:
The α-Helical Cap Domain of a Novel Esterase from Gut Alistipes shahii Shaping the Substrate-Binding Pocket.
Wei X, Wang YL, Wen BT, Liu SJ, Wang L, Sun L, Gu TY, Li Z, Bao Y, Fan SL, Zhou H, Wang F, Xin F
J Agric Food Chem 69 6064-6072 (2021)
PMID: 33979121

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 266 amino acids
Theoretical weight: 29.54 KDa
Source organism: Alistipes shahii WAL 8301
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D4INH0 (Residues: 1-266; Coverage: 100%)
Gene name: AL1_21970
Sequence domains: Alpha/beta hydrolase family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 45.469Å b: 75.545Å c: 161.021Å
α: 90° β: 92.34° γ: 90°
R-values:
R R work R free
0.168 0.166 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Characterization of feruloyl esterases from Pecoramyces sp. F1 and the synergistic effect in biomass degradation.
Ma et al. (2022)
1 more