› 7ds3

X-ray diffraction
2.09Å resolution

Crystal structure of actin capping protein in complex with twinflin-2 C-terminus tail

Released:
DOI: 10.2210/pdb7ds3/pdb
PDB entry 7ds3 coloured by chain, front view.
PDB entry 7ds3 coloured by chain, side view.
PDB entry 7ds3 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Insights into the Regulation of Actin Capping Protein by Twinfilin C-terminal Tail.
Takeda S, Koike R, Fujiwara I, Narita A, Miyata M, Ota M, Maéda Y
J Mol Biol 433 166891 (2021)
PMID: 33639213

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-146484 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
F-actin-capping protein subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 33 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13127 (Residues: 1-286; Coverage: 100%)
Gene name: CAPZA1
Sequence domains: F-actin capping protein alpha subunit
F-actin-capping protein subunit beta isoforms 1 and 2 Chain: B
Molecule details ›
Chain: B
Length: 244 amino acids
Theoretical weight: 27.47 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14315 (Residues: 1-244; Coverage: 88%)
Gene name: CAPZB
Sequence domains: F-actin capping protein, beta subunit
Twinfilin-2 Chain: C
Molecule details ›
Chain: C
Length: 30 amino acids
Theoretical weight: 3.29 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q9Z0P5 (Residues: 315-344; Coverage: 9%)
Gene names: Ptk9l, Twf2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AichiSR BEAMLINE BL2S1
Spacegroup: P212121
Unit cell:
a: 51.51Å b: 67.64Å c: 164.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 citation in other articles

The Eukaryotic Linear Motif resource: 2022 release.
Kumar et al. (2022)
2 more