› 7dsa

X-ray diffraction
2.8Å resolution

Crystal structure of actin capping protein in complex with V-1 (space group P62)

Released:
DOI: 10.2210/pdb7dsa/pdb
PDB entry 7dsa coloured by chain, front view.
PDB entry 7dsa coloured by chain, side view.
PDB entry 7dsa coloured by chain, top view.
Source organisms:
Primary publication:
Structural Insights into the Regulation of Actin Capping Protein by Twinfilin C-terminal Tail.
Takeda S, Koike R, Fujiwara I, Narita A, Miyata M, Ota M, Maéda Y
J Mol Biol 433 166891 (2021)
PMID: 33639213

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-146475 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
F-actin-capping protein subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 33 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13127 (Residues: 1-286; Coverage: 100%)
Gene name: CAPZA1
Sequence domains: F-actin capping protein alpha subunit
F-actin-capping protein subunit beta isoforms 1 and 2 Chain: B
Molecule details ›
Chain: B
Length: 244 amino acids
Theoretical weight: 27.47 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14315 (Residues: 1-244; Coverage: 88%)
Gene name: CAPZB
Sequence domains: F-actin capping protein, beta subunit
Myotrophin Chain: C
Molecule details ›
Chain: C
Length: 123 amino acids
Theoretical weight: 13.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P58546 (Residues: 1-118; Coverage: 100%)
Gene name: MTPN
Sequence domains: Ankyrin repeats (3 copies)

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AichiSR BEAMLINE BL2S1
Spacegroup: P62
Unit cell:
a: 160.293Å b: 160.293Å c: 51.999Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.213 0.254
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

The Eukaryotic Linear Motif resource: 2022 release.
Kumar et al. (2022)
2 more