› 7dzv

X-ray diffraction
1.6Å resolution

Cyrstal structure of PETase E186A mutant from Rhizobacter gummiphilus

Released:
DOI: 10.2210/pdb7dzv/pdb
PDB entry 7dzv coloured by chain, front view.
PDB entry 7dzv coloured by chain, side view.
PDB entry 7dzv coloured by chain, top view.
Source organism: Rhizobacter gummiphilus
Primary publication:
Implications for the PET decomposition mechanism through similarity and dissimilarity between PETases from Rhizobacter gummiphilus and Ideonella sakaiensis.
Sagong HY, Son HF, Seo H, Hong H, Lee D, Kim KJ
J Hazard Mater 416 126075 (2021)
PMID: 34492896

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DLH domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 28.48 KDa
Source organism: Rhizobacter gummiphilus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1W6L588 (Residues: 27-292; Coverage: 100%)
Gene name: A4W93_05950
Sequence domains: Dienelactone hydrolase family

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 50.988Å b: 54.627Å c: 108.887Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.133 0.157
Expression system: Escherichia coli

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Citations

3 review citations

Current advances in the structural biology and molecular engineering of PETase.
Liu et al. (2023)
2 more